![]() ![]() ![]() Hence, quantitatively identical bacterial loads with mono- or polymicrobial dominance lead to a different degree of sepsis severity and divergent outcomes. Furthermore, we found a relationship between ascites CFUs and the evolving change in ROFA scores throughout the study. ROFA scores dropped after 48 h, accompanied by a steady decrease in ascites CFUs and a shift towards intra-abdominal monomicrobial E. coli suspension, and ROFA scores were significantly higher in the monomicrobial subgroup than in the polymicrobial one (median 6.5 5.0–7.0 and 5.0 4.75–5.0, respectively). At this time, 20% mortality occurred in animals receiving a monomicrobial E. ![]() ![]() Invasive monitoring was conducted in separate anesthetized groups (n = 12–13/group) after 12, 24, 48 and 72 h to determine rat-specific organ failure assessment (ROFA) scores. The qualitative composition of the initial inoculum and the ascites was analyzed separately by MALDI-TOF mass spectrometry. Saline or fecal suspensions with a standardized dose range of bacterial colony-forming units (CFUs) were injected intraperitoneally into Sprague–Dawley rats. We hypothesized that the composition of sepsis-inducing bacterial flora influences the course of fecal peritonitis in rodents. ![]()
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